Professional Excel tool for comparability verification between quantitative methods and analyzers according to ISO 15189:2022 and CLSI EP09. Includes automatic calculation of bias, regression, correlation, Mean Bias (%), SD, R² and automatic PASS / FAIL interpretation. Ideal for accredited medical laboratories or laboratories preparing for accreditation.


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This professional Excel tool was developed for medical laboratories that need to perform comparability verification between two quantitative analytical methods or two analyzers, according to ISO 15189:2022 requirements and CLSI EP09 recommendations.

The tool allows evaluation of analytical differences between two measurement systems using real patient samples and automatically generates the essential statistical parameters required for comparability assessment.

The Excel file is designed for practical use in accredited medical laboratories or laboratories preparing for accreditation and can be used both for internal verification studies and audit documentation.


What Can This Tool Be Used For

This Excel tool can be used for:

  • implementation of a new analyzer
  • comparison between two analyzers within the same laboratory
  • comparison between analyzers from different laboratory locations
  • reagent lot or reagent system changes
  • post-maintenance or recalibration verification
  • comparison between an internal method and a reference method
  • harmonization assessment between analytical systems
  • demonstrating comparability according to ISO 15189 requirements

Automatic Calculations Included

After entering the results into the highlighted cells, the Excel file automatically calculates:

Individual Parameters

  • difference between methods
  • bias (%) for each sample
  • average of paired results

Global Statistical Parameters

  • Mean Method 1
  • Mean Method 2
  • Mean Difference
  • Mean Bias (%)
  • Standard Deviation (SD)
  • Correlation Coefficient (r)
  • Regression Slope
  • Regression Intercept
  • Coefficient of Determination (R²)

Automatic PASS / FAIL Evaluation

The file automatically compares Mean Bias (%) with the user-defined acceptance criterion and provides automatic interpretation:

  • PASS = acceptable comparability
  • FAIL = unacceptable comparability

Key Features

1. Professional Structure

The Excel file is clearly organized and intuitive:

  • complete instructions included
  • highlighted data entry areas
  • protected calculation formulas
  • automatic interpretation
  • optimized design for practical laboratory use

2. Minimum Sample Number According to CLSI EP09

The file is configured for:

  • recommended minimum: 40 samples
  • coverage of low / normal / high concentration ranges
  • possibility of using duplicate patient samples when sample availability is limited

3. Compatible with Multiple Laboratory Areas

Suitable for:

  • clinical chemistry
  • immunology
  • hematology
  • tumor markers
  • hormones
  • quantitative serological assays
  • coagulation
  • other quantitative methods

Advantages of Using This Excel Tool

Major Time Saving

Eliminates manual calculations and significantly reduces the time required for comparability verification.

Reduced Risk of Errors

All statistical formulas are already implemented and verified.

Standardization

Provides a uniform and reproducible approach for method comparison studies.

Useful for Audit and Accreditation

Can be used as supporting documentation for:

  • ISO 15189 accreditation
  • internal audits
  • external audits
  • method verification studies
  • quality management system documentation

Easy to Use

No additional statistical software is required.

Simply enter the results into the Excel file.


Target Users

This product is intended for:

  • laboratory physicians
  • biologists
  • biochemists
  • chemists
  • quality managers
  • laboratory managers
  • ISO 15189 accredited laboratories
  • laboratories preparing for accreditation

What the Product Includes

The package includes:

  • fully automated Excel file
  • instructions for use included in the workbook
  • automatic statistical interpretation
  • PASS / FAIL evaluation
  • ready-to-use professional structure

References Used

This tool was developed based on the following standards and recommendations:

  • ISO 15189:2022
  • CLSI EP09
  • statistical principles for quantitative method comparison
  • recommendations regarding bias and comparability assessment

Benefits for the Laboratory

By using this Excel tool, the laboratory can:

  • demonstrate comparability between analytical systems
  • document verification studies required for accreditation
  • rapidly identify proportional or constant errors
  • evaluate inter-method bias
  • standardize comparison procedures
  • reduce time spent on statistical analysis

Important Notes

  • This file is intended exclusively for quantitative methods.
  • Final interpretation must be performed by qualified personnel.
  • The laboratory should define its own acceptance criteria before starting the study.
  • The use of real patient samples is strongly recommended.

Compatibility

  • Microsoft Excel
  • Windows compatible
  • No complex macros or additional software required

Ideal for Laboratories Seeking

✔ fast and professional verification studies

✔ audit-ready documentation

✔ ISO 15189 compliant standardization

✔ reduced statistical calculation time

✔ automatic and clear interpretation

✔ simple implementation into daily laboratory practice

Disclaimer

This Excel tool is intended exclusively for professional use by qualified laboratory personnel. The calculations and statistical interpretations provided by the file are designed to support method comparison and comparability verification activities in medical laboratories according to ISO 15189 and CLSI EP09 recommendations.

The final interpretation of results, acceptance criteria, and validation decisions remain the sole responsibility of the laboratory and its authorized personnel.

This product does not replace professional judgment, regulatory requirements, manufacturer instructions, or laboratory-specific procedures.

The author assumes no liability for decisions, actions, or consequences resulting from the use or misuse of this file.

Users are responsible for verifying the suitability of the tool for their specific laboratory applications and regulatory environment.


Copyright Notice

© Dr. Valeria Mureșan – All Rights Reserved.

This Excel tool, including its structure, formulas, statistical calculations, design, instructions, and accompanying materials, is protected by copyright law.

Unauthorized reproduction, redistribution, resale, modification, sharing, publication, or commercial use of this product, in whole or in part, is strictly prohibited without prior written permission from the author.

The purchase of this product grants a non-transferable license for use within a single laboratory or organization only.

For collaborations, institutional licenses, or distribution permissions, please contact Dr. Valeria Mureșan.